Ribonucleic acid (RNA)is a versatile molecule, thus the opportunity to target RNA is abundant. Hence, the currentexploration deals with the interaction of a metal complex of hydroxamic acid with t-RNA, along with HDAC8 inhibition activity and antioxidant activity. Binding characteristics of complex1, Bis(N-phenylbenzohydroxamato)Tungsten(VI), which is represented as [N-PBHA-W(VI)] with Torula yeast RNA (t-RNA) were investigated by using several types of spectroscopic techniques, measurements of viscosity, and computational study through molecular docking. The intrinsic binding constant, Kb was evaluated for complex 1which shows changes in the shift of absorption spectra,and the intensity of spectra also varies. From fluorescence measurement,the binding constant of complex 1towards t-RNA is 6.64 ± 0.05 × 105 M−1. Stern-Volmer quenching constant was calculated. Two displacement methodsare performed by using the fluorescence spectroscopic technique which discloses a groove mode of binding. The groove mode of binding is also uncovered by viscosity measurement, which was observed by increasing the complex 1 concentration. An electrochemical investigation was carried out by using cyclic voltammetry. A circular dichroism study was also employed,which further suggests a strong binding occurred between the metal complex used and t-RNA. The docked postures of t-RNA with complex 1expose the strong interactions which suggesta minor groove mode of binding. All the experimental evidence indicates that the interaction of complex 1 with t-RNA is a minor groove mode of binding which complements the molecular docking results. In-silico studies of HDAC8 inhibition activity ofcomplex 1 reveal that the inhibition takes place mainly by hydrophobic interaction. DPPH–radical scavenging method employed for the antioxidant activity.